rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Transfer and expression of a retrovirus vector containing an interferon-alpha1 gene in interferon deficient cultured cells
  name:
    name part: Dilling, Michael Bruce.
    role:
      role term: creator
  name:
    name part: Collisson, Ellen W.
    role:
      role term: degree supervisor.
  name:
    name part: Kraemer, Duane C.
    role:
      role term: degree supervisor.
  name:
    name part: Skow, Loren C.
    role:
      role term: degree committee member.
  name:
    name part: Womack, James E.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1993
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 218 leaves : illustrations ; 28 cm
  abstract: The possibility of using retrovirus-mediated gene transfer to interferon-deficient cells as a model system for examining expression differences between members of the interferon-α gene family was investigated. A retrovirus vector containing an interferon-α1 gene was constricted and used to transfer this interferon gene to cultured cell lines bearing interferon gene deletions. An African green monkey genomic interferon-al gene was inserted into the cloning site of the retrovirus vector B2N and the orientation of the inserted gene was confirmed by DNA sequencing. B2N is a retrovirus vector designed for the expression of sequences under the control of an internal promoter supplied with the gene of interest. The interferon gene containing vector was packaged into infectious virions using the amphotropic packaging cell line ψ-CRIP. Recombinant virions were titered by infection of murine NIH/3T3 cells and titers ranged from 1 to 3.73x10^5 colony forming units/ml of virus supernatant. Delivery of correct proviral structure was confirmed by Southern blotting. Two interferon deficient cultured cell lines, the human chronic myelogenous leukemia K562 line, and the African green monkey Vero line, were successfully infected with the interferon gene containing vector as determined by Southern blotting. Clones of infected cells were isolated and analyzed for expression of vector sequences using cytopathic effect reduction bioassays, RNase protection assays, and Northern blotting. Expression of the transferred interferon gene was induced by infection with Bluetongue virus (BTV), a potent interferon inducing agent. Northern blotting showed expression of the vector at the RNA level. Quantitative changes in expression levels were seen in BTV-induced Vero cells. Attempts to demonstrate quantitative changes in expression over a time course after BTV induction showed variable expression, however. Cytopathic effect reduction assays showed no expression of biologically active interferon. T…
  note: Vita.
  note: "Major subject: Genetics."
  note: In title, symbol for Greek letter alpha is used.
  note: Includes bibliographical references.
  note: Ph. D. Texas A & M University 1993
  subject:
    topic: Gene expression
  subject:
    topic: Interferon
  subject:
    topic: Eukaryotic cells
  subject:
    topic: Cytogenetics
  subject:
    topic: Major genetics
  identifier: http://gateway.proquest.com/openurl
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 960319
    record change date: 20200904152653.0
    record identifier: in00001258558
    language of cataloging:
      language term: eng