Nonisotopic probing, blotting, and sequencing /

Bibliographic Details
Other Authors:	Kricka, Larry J., 1947-
Format:	Book
Language:	English
Published:	San Diego : Academic Press, [1995]
Edition:	2nd ed.
Subjects:	Molecular probes. Immunoblotting. Chemiluminescence assay. Bioluminescence assay. Amino acid sequence. Nucleotide sequence.

Table of Contents:

Pt. 1. Introduction : Labels, labeling, analytical strategies, and applications
Methods for nonradioactive labeling of nucleic acids. Pt. 2. Detection methods : Detection of alkaline phosphatase labels by time-resolved fluorescence
Detection of alkaline phosphatase by bioluminescence
Detection of DNA on membranes with alkaline phosphatase-labeled probes and chemiluminescent CSPD[superscript R]substrate
Detection of alkaline phosphatase by colorimetry
Detection of alkaline phosphatase by chemiluminescence using NADP, ascorbate, and indolyl phosphates
Detection of horseradish peroxidase by enhanced chemiluminescence
Detection of horseradish peroxidase by colorimetry
Detection of glucose-6-phosphate dehydrogenase by bioluminescence
Detection of xanthine oxidase by enhanced chemiluminescence
Electrochemiluminescent detection of PCR-derived nucleic acids
Detection of phosphors by phosphorescence
Detection of europium cryptates by time-resolved fluorescence
Detection of lanthanide chelates by time-resolved fluorescence
Detection of lanthanide chelates and multiple labeling strategies based on time-resolved fluorescence
Detection of acridinium esters by chemiluminescence
Detection of energy transfer and fluorescence quenching. Pt. 3. DNA sequencing : DNA sequencing by nonisotopic methods
Chemiluminescent DNA sequencing with 1,2-dioxetanes.