rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Somatic cell hybrids
    sub title: the basics
  name:
    name part: Abbott, Catherine M.
    role:
      role term: creator
  name:
    name part: Povey, S. (Sue)
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: enk
    date issued: 1995
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: ix, 76 pages : illustrations ; 25 cm.
  table of contents: Why do we map genes -- What are somatic cell hybrids -- Uses of somatic cell hybrids -- How do we map genes -- How else can somatic cell hybrids be of use -- Monochromosomal hybrids -- Gene expression studies -- Studies of malignancy -- Where do I begin -- Where do I find out more -- Making somatic cell hybrids -- Characteristics of different cultured cells -- Primary diploid cultures -- Permanent cell lines -- EBV-transformed lymphoblastoid lines -- Permanent rodent cell lines -- Induction of mutations in permanent cell lines -- HPRT -- Thymidine kinase (TK).
  table of contents: Selection of hybrid cells -- HAT selection -- Cell fusion -- Fate of hybrid cells and expansion of the culture -- Making a hybrid panel -- Characterizing somatic cell hybrids -- Karyotyping -- Methods of analysis -- Counting -- Giemsa-banding (G-banding) -- Fluorescent in situ hybridization and hybrid chromosomes -- Reverse karyotyping -- Other hybrids -- Isoenzymes -- Methods -- Advantages -- Disadvantages -- Southern blotting -- Polymerase chain reaction (PCR) -- Introduction -- Methodology -- Mapping genes using somatic cell hybrids.
  table of contents: Introduction: the concept of a mapping panel -- Which technique to use for mapping -- Mapping genes using southern blotting -- Choice of probe -- Probes which detect variable loci -- Washing stringency -- Mapping genes using PCR -- Some problems commonly encountered -- Chromosome fragmentation -- Pseudogenes -- Southern blotting -- PCR -- Modification of the basic techniques: designer hybrids -- Selection of chromosomes other than X or 17 -- Mutation complementation -- Selection of genes coding the surface antigens -- Selection for oncogenes.
  table of contents: Dominant selectable markers -- Gene targeting -- Hybrids containing single chromosomes -- Microcell hybrids -- Hybrids containing parts of chromosomes -- Chromosome-mediated gene transfer (CMGT) -- Irradiation-fusion gene transfer (IFGT) -- Telomere-associated chromosome fragmentation -- Other uses of hybrids -- Direct isolation of disease genes -- Generation of region-specific probes from hybrids -- IRS-PCR -- Identification of expressed sequences from hybrids -- Differentiation -- Malignancy -- Hybridomas.
  note: C. Abbott and S. Povey.
  note: Includes bibliographical references and index.
  subject:
    topic: Somatic hybrids
  subject:
    topic: Gene mapping
    topic: Methodology
  classification: QH451 .A22 1995
  classification: 574.87/322
  identifier: 0199634432 :
  identifier: 94019265
  record info:
    record content source: DLC
    record creation date: 940516
    record change date: 20151003063205.0
    record identifier: in00001229574