http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Why do we map genes -- What are somatic cell hybrids -- Uses of somatic cell hybrids -- How do we map genes -- How else can somatic cell hybrids be of use -- Monochromosomal hybrids -- Gene expression studies -- Studies of malignancy -- Where do I begin -- Where do I find out more -- Making somatic cell hybrids -- Characteristics of different cultured cells -- Primary diploid cultures -- Permanent cell lines -- EBV-transformed lymphoblastoid lines -- Permanent rodent cell lines -- Induction of mutations in permanent cell lines -- HPRT -- Thymidine kinase (TK).
Selection of hybrid cells -- HAT selection -- Cell fusion -- Fate of hybrid cells and expansion of the culture -- Making a hybrid panel -- Characterizing somatic cell hybrids -- Karyotyping -- Methods of analysis -- Counting -- Giemsa-banding (G-banding) -- Fluorescent in situ hybridization and hybrid chromosomes -- Reverse karyotyping -- Other hybrids -- Isoenzymes -- Methods -- Advantages -- Disadvantages -- Southern blotting -- Polymerase chain reaction (PCR) -- Introduction -- Methodology -- Mapping genes using somatic cell hybrids.
Introduction: the concept of a mapping panel -- Which technique to use for mapping -- Mapping genes using southern blotting -- Choice of probe -- Probes which detect variable loci -- Washing stringency -- Mapping genes using PCR -- Some problems commonly encountered -- Chromosome fragmentation -- Pseudogenes -- Southern blotting -- PCR -- Modification of the basic techniques: designer hybrids -- Selection of chromosomes other than X or 17 -- Mutation complementation -- Selection of genes coding the surface antigens -- Selection for oncogenes.
Dominant selectable markers -- Gene targeting -- Hybrids containing single chromosomes -- Microcell hybrids -- Hybrids containing parts of chromosomes -- Chromosome-mediated gene transfer (CMGT) -- Irradiation-fusion gene transfer (IFGT) -- Telomere-associated chromosome fragmentation -- Other uses of hybrids -- Direct isolation of disease genes -- Generation of region-specific probes from hybrids -- IRS-PCR -- Identification of expressed sequences from hybrids -- Differentiation -- Malignancy -- Hybridomas.
Includes bibliographical references and index.