rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: use of H+-ATP synthase for regenerating ATP in synthetic enzymatic reactions
  name:
    name part: Nam, Ki Yun.
    role:
      role term: creator
  name:
    name part: Holtzapple, Mark T.
    role:
      role term: degree supervisor.
  name:
    name part: Hanson, Daniel T.
    role:
      role term: degree committee member.
  name:
    name part: Struck, Douglas K.
    role:
      role term: degree committee member.
  name:
    name part: White, Ralph E.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1992
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: x, 174 leaves : illustrations ; 28 cm
  abstract: Application of enzymes to industrial processes has been limited to degradative reactions, since many enzymatic syntheses require hydrolysis of stoichiometric amounts of ATP (adenosine triphosphate) to drive the reactions. Since ATP is expensive, it is necessary to regenerate ATP from ADP during the course of the reaction. Enzymatic regeneration from chemically synthesized high-energy phosphate donors, the best scheme currently available, has the major disadvantage in that the product produced from the phosphate donor accumulates and is not easily removed. In this research, we investigated whether the thermophilic ATP synthase (TF[0]F[1]) from the bacterium PS3 was suitable for ATP regeneration. This enzyme can synthesize ATP without producing waste products (i.e., spent donors) since the reaction is driven by a pH gradient provided by an acid-base change. PS3 synthase was selected since it is stable compared to other ATP synthases. TF[0]F[1] was purified from the thermophilic bacterium PS3 and reconstituted into liposomes by the cholate-dialysis method. The reconstituted TF[0]F[1] liposomes showed synthetic activity of 10-20 nmol ATP/mg TF[0]F[1] by an acid-base change when ATP was captured with hexokinase. Higher amounts of ATP (maximum of 60 nmol ATP/mg TF[0]F[1] could be synthesized when ATP was captured with glycerokinase. The fluorescence quenching assay after repeated acid-base changes showed that TF[0]F[1] liposomes did not lose proton-pumping activity even after six acid-base changes performed over a 2-week period. These preliminary results indicate that ATP can be synthesized by repeated acid-base changes. A modeling study of a large-scale ATP-regeneration scheme showed that significantly less energy is required if the liposomes are immobilized on glass spheres rather than used in aqueous suspension. In this scheme, the KOH and HCl required to produce the pH gradient are regenerated from KCl by water-splitting electrodialysis technology. Calculations show t…
  note: Vita.
  note: "Major subject: Chemical Engineering."
  note: Includes bibliographical references.
  note: Ph. D. Texas A & M University 1992
  subject:
    topic: Adenosine triphosphate
    topic: Synthesis
  subject:
    topic: Adenosine triphosphatase
  subject:
    topic: Enzymatic analysis
  subject:
    topic: Major chemical engineering
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 950227
    record change date: 20200904152631.0
    record identifier: in00001222409
    language of cataloging:
      language term: eng