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Techniques in protein modification /

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Bibliographic Details
Main Author: Lundblad, Roger L.
Format: Book
Language:English
Published: Boca Raton, Fla. : CRC Press, [1995]
Subjects:
Proteins > Chemical modification.
Protein Processing, Post-Translational.
Proteins > analysis.
Indicators and Reagents.
Table of Contents:
  • Site-specific chemical modification of proteins
  • Amino acid analysis
  • Peptide separation by reverse-phase high-performance liquid chromatography
  • Methods for sequence determination
  • Chemical cleavage of peptide bonds
  • Modification of cysteine
  • Modification of cystine: cleavage of disulfide bonds
  • Modification of methionine
  • Modification of histidine residues
  • Modification of lysine
  • Modification of arginine
  • Chemical modification of tryptophan
  • Modification of tyrosine
  • Modification of carboxyl groups.
  • Chemical cross-linking of peptide chains
  • Some commonly used reagents for site-specific modification in proteins
  • Assay of protein concentration in solution.