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The Polymerase chain reaction /

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Bibliographic Details
Other Authors: Mullis, Kary, Ferré, François, Gibbs, Richard (Richard A.)
Format: Book
Language:English
Published: Boston : Birkhäuser, [1994]
Subjects:
Polymerase chain reaction.
Polymerase Chain Reaction.
Table of Contents:
  • Methodology
  • Basic methodology
  • Manipulation of DNA by PCR / Kenshi Hayashi
  • Cloning PCR products / Michael A. Frohman
  • Optimization of multiplex PCRs / Jeffrey S. Chamberlain and Joel R. Chamberlain
  • Preparation of nucleic acids for archival material / Darryl Shibata
  • PCR amplification of viral DNA and viral host cell mRNAs in situ / Janet Embretson ... [et al.]
  • Quantitation
  • Quantitative PCR: an overview / F. Ferre ... [et al.]
  • Quantification of DNAs by the polymerase chain reaction using an internal control / Donald M. Coen
  • RT-PCR and mRNA quantitation / Jamel Chelly and Axel Kahn
  • Analysis of human T-cell repertoires by PCR / Dominic G. Spinella and James M. Robertson
  • Nonisotopic detection
  • Ultrasensitive nonradioactive detection of PCR reactions: an overview / Richard H. Tullis
  • Fluorescent detection methods for PCR analysis / Michael J. Heller
  • Enzyme-labeled oligonucleotides / Eugene Tu and Edward Jablonski
  • Application of the hybridization protection assay (HPA) to PCR / Norman C. Nelson and Sherrol H. McDonough.
  • (cont) Instrumentation
  • PCR instrumentation: where do we stand? / Christian C. Oste
  • Rapid cycle DNA amplification / Carl T. Wittwer, Gudrun B. Reed and Kirk M. Ririe
  • Automating the PCR process / H.R. Garner
  • Sequencing
  • PCR and DNA sequencing / Bjorn Andersson and Richard A. Gibbs
  • Phage promoter-based methods for sequencing and screening for mutations / Steve S. Sommer and Erica L. Vielhaber
  • Capture PCR: an efficient method for walking along chromosomal DNA and cDNA / Andre Rosenthal, Matthias Platzer and D. Stephen Charnock-Jones
  • Applications
  • General applications
  • In vitro evolution of functional nucleic acids: high-affinity RNA ligands of the HIV-1 rev protein / Craig Tuerk ... [et al.]
  • Application of PCR to forensic science / Bruce Budowle ... [et al.]
  • Recreating the past by PCR / Matthias Hoss, Oliva Handt and Svante Paabo
  • Nonbiological applications / Gavin Dollinger
  • Genetic analysis
  • RT-PCR and gene expression / Didier Montarras ... [et al.].
  • (cont) Fingerprinting using arbitrarily primed PCR: application to genetic mapping, population biology, epidemiology and detection of differentially expressed RNAs / John Welsh and Michael McClelland
  • Genetics, plants and the polymerase chain reaction / Bruno W.S. Sobral and Rhonda J. Honeycutt
  • Assessment of therapy effectiveness
  • PCR assessment of the efficacy of therapy in Philadelphia chromosome-positive leukemias / Stephen P. Hunger and Michael L. Cleary
  • Detection of minimal residual disease (MRD) in acute lymphoblastic leukemia using clone-specific probes directed against V(D)J junctional sequences / Luc d'Auriol and Francois Sigaux
  • Assessment of therapy effectiveness: infectious disease / Salvatore J. Arrigon
  • Gene therapy / Richard A. Morgan and W. French Anderson
  • Diagnostics
  • PCR and cancer diagnostics: detection and characterization of single point mutations in oncogenes and antioncogenes / Manuel Perucho.
  • (cont) Clinical applications of the polymerase chain reaction / Belinda J.F. Rossiter and C. Thomas Caskey
  • Infectious disease / W. John Martin
  • PCR and scientific invention: the trial of DuPont vs. Cetus / Kary B. Mullis.