Immunochemical protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1992]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
10. |
| Subjects: |
Table of Contents:
- Production of polyclonal antisera
- Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogens
- Raising polyclonal antibodies using nitrocellulose-bound antigen
- Synthesis of peptides for use as immunogens
- Production and characterization of antibodies against synthetic peptides
- Preparation and testing of monoclonal antibodies to recombinant proteins
- Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase
- Purification of immunoglobulin G (IgG)
- Epitope mapping
- Enzyme-antienzyme method for immunohistochemistry
- Double label immunohistochemistry on tissue sections using alkaline phosphatase and peroxidase conjugates
- Immunohistochemical detection of bromodeoxyuriding-labeled nuclei for in vivo cell kinetic studies
- Avidin-biotin technology: preparation of biotinylated probes
- Avidin-biotin technology: preparation of avidin conjugates
- Immunochemical applications of avidin-biotin technology
- Preparation of gold probes.
- (cont.) Immunogold probes for light microscopy
- Immunogold probes in electron microscopy
- Electron microscopic silver enhancement for double labeling with antibodies raised in the same species
- Quantitative and qualitative immunoelectrophoresis: general comments on principles, reagents, equipment, and procedures
- Rocket immunoelectrophoresis techniques or electroimmunodiffusion
- Crossed immunoelectrophoresis
- Crossed immunoaffinoelectrophoresis
- Immunodetection of proteins by western blotting
- Erasable western blots
- Colloidal gold staining and immunoprobing on the same western blot
- Colloidal gold staining and immunodetection in 2-D protein mapping
- Fluorescent protein staining on nitrocellulose with subsequent immunodetection of antigen
- Competitive ELISA
- Twin-site ELISAs for fos and myc oncoproteins using the AMPAK system
- Preparation of cytotoxic antibody-toxin conjugates
- Immunoaffinity purification and quantification of antibody-toxin conjugates
- An immuno-slot-blot assay for detection and detection and quantitation of alkyldeoxyguanosines in DNA.
- (cont.) Production of monoclonal antibodies for the detection of chemically modified DNA
- Sensitive competitive enzyme-linked immunoassay for quantitation of modified bases in DNA
- An immunochemical assay for detecting transition of B-DNA to Z-DNA
- Cell sorting using immunomagnetic beads
- Cell preparation for flow cytometry
- Preparation of rat lung cells for flow cytometry
- The isolation of rat hepatocytes for flow cytometry
- Flow cytometric analysis of cells using an antibody to a surface antigen
- Multiple immunofluorescence analysis of cells using flow cytometry
- Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine
- Production and use on nonradioactive hybridization probes
- Cellular human and viral DNA detection by nonisotopic in situ hybridization
- Chromosomal mapping of genes by nonisotopic in situ hybridization
- Nonisotopic in situ hybridization: immunocytochemical detection of specific repetitive sequences on chromosomes and interphase nuclei
- Biotinylated probes in colony hybridization.
- (cont.) Screening of agt11 cDNA libraries using monoclonal antibodies
- Expression of foreign genes in mammalian cells using an antibody fusion system.