Molecular and pathogenesis studies of Cache Valley virus : a common teratogenic agent in sheep /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1992.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | Serological data suggested that Cache Valley virus was the agent that caused an outbreak of congenital malformations in neonatal lambs in San Angelo, Texas in 1986- 1987 lambing season. During that lambing season, a total of 69 (19.2%) of 360 new born lambs were characterized as arthrogryposis-hydranencephaly. Similar clinical signs were observed in fetal sheep when inoculated in utero with a strain of Cache Valley virus, which was isolated in the San Angelo, Texas area. A cDNA corresponding to the full length of the small RNA genome segment of Cache Valley virus was synthesized, cloned, sequenced, and expressed. This segment of RNA contains 949 bases. Computer analyses indicated that this segment had 3 open reading frames including the nucleocapsid protein gene of 702 bases and two nonstructural protein genes with 114 bases and 153 bases. The nucleocapsid gene was subcloned and the nucleocapsid protein was successfully expressed in 2 prokaryotic expression systems. The cDNA has been successful used as a probe to detect the small RNA segment of Cache Valley virus in Vero cells. The cDNA probe and the antiserum to the nucleocapsid protein would be a good combination for the study of teratogenesis of Cache Valley virus in fetal sheep. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Veterinary Microbiology." |
| Physical Description: | xiv, 119 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |