rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Production kinetics of immunosuppressive fungal metabolites
  name:
    name part: Mateu, Sylvie
    name part: 1962-
    role:
      role term: creator
  name:
    name part: Engler, Cady R.
    role:
      role term: degree supervisor.
  name:
    name part: Garcia, Albert
    role:
      role term: degree supervisor.
  name:
    name part: Kenerley, Charles M
    role:
      role term: degree committee member.
  name:
    name part: Sweeten, John M.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses.
  origin info:
    place:
      place term: xx
    date issued: 1992
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xvii, 180 leaves : illustrations ; 29 cm
  abstract: Artificial suppression of the immune system is vital to organ transplant therapy as well as treatment of auto-immune diseases. Unfortunately few non-toxic immunosuppressive drugs exist. In search for such pharmaceuticals with low toxicity, this work offers a preliminary investigation into characterization and optimization of production of compounds found recently to have immunosuppressive activity. One fungal strain from each of three genera (Emericella, Paecilomyces and Penicillium), produced bioactive metabolites in separate bioreactor fermentations. Preliminary screening showed that Penicillium produced citrinin, a known toxic compound. The two other strains produced an immunosuppressive metabolite. The unknown immunosuppressive metabolite was extracted from Emericella broth and characterized by HPLC and TLC. Results allowed comparison to known metabolites, suggesting vertisporin and especially vermiculin as likely candidates. More complete characterization by mass spectrometry requires pure samples. Several methods of purification were tried including HPLC, TLC, and a combination of membrane filtration and size-exclusion chromatography. Unfortunately, separation was impeded by depletion of the stock of bioreactor fermentation broth and deterioration of the acid extract. To circumvent these problems, fresh fermentation broth was produced on a regular basis in shake flasks. Thus, bioactive samples were available throughout the entire separation experiment. But broths from shake-flask fermentations did not contain the immunosuppressive metabolite although they did contain up to three metabolites with antibacterial activity, two of which were likewise produced in the bioreactor. One antibacterial metabolite produced in both types of fermentations was purified sufficiently to be characterized by mass spectrometry. Although not fully identified, its characteristics suggest that it may be a metabolic precursor of the immunosuppressive metabolite..
  note: by Sylvie Mateu.
  note: "Major subject: Agricultural Engineering."
  note: Typescript (photocopy).
  note: Vita.
  note: Ph. D. Texas A & M University 1992
  note: Includes bibliographical references.
  subject:
    topic: Fungal metabolites
  subject:
    topic: Immunosuppression
  subject:
    topic: Microbiological synthesis
  subject:
    topic: Agricultural Engineering
  subject:
    topic: Major agricultural engineering
  subject:
    topic: Fungal metabolites
  subject:
    topic: Immunosuppression
  subject:
    topic: Microbiological synthesis
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-1354141
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-1354141
  record info:
    record content source: TXA
    record creation date: 931008
    record change date: 20220103134954.0
    record identifier: in00001160536
    language of cataloging:
      language term: eng