The metabolism of [12, 13-3H]c̲i̲s̲-12-octadecenoic acid in rat liver and adipose tissue lipids /
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| Other Authors: | , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1991.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | cis-12-Octadecenoic acid is one of the octadecenoic acid isomers found in partially hydrogenated vegetable oils. This isomer is found in both human and animal lipids. The extent of incorporation and metabolism of a synthetic [12,13-[^3]H] cis-12-octadecenoic acid was investigated. Fifteen male Sprague Dawley rats were intragastically fed 46 μCi of the (12,13-[^3]H) cis-12-octadecenoic acid. Thirty minutes and 2, 4, 8, and 18 hours after intubation, 3 rats were sacrificed. Lipids of liver and adipose tissues were isolated and the incorporation of (12,13-[^3]H) cis-12-octadecenoic acid was analyzed. The data indicated that a higher percentage of (12,13-[^3]H) cis-12-octadecenoic acid was found in liver tissue lipids than adipose tissue lipids. (12,13-[^3]H) cis-12-Octadecenoic acid incorporation into liver phospholipid was greatest at 30 min, then progressively declined over time. The amount of radioactivity found in the liver tissue ranged from 18.5 to 30 nCi per gram tissue. However, the level of radioactivity in the adipose tissue lipids never exceed 21.4 nCi. (12,13-[^3]H) cis-12-Octadecenoic acid was incorporated into all of the liver phospholipid classes. The extent of incorporation into phosphatidylcholine and phosphatidylethanolamine was approximately the same. The amount of radioactivity found in these two fractions was about 75% of the total activity found in the liver phospholipids. The majority of the (12,13-[^3]H) cis-12-octadecenoic acid in the adipose tissue lipids was found in the triglyceride fraction. (12,13-[^3]H) cis-12-Octadecenoic acid was desaturated to a octadecadienoic acid. However, the actual product could not be determined. It was speculated that one of the product could be the essential fatty acid, cis-9, cis-12-octadecadienoic acid. These data indicated that (12,13-[^3]H) cis-12-octadecenoic acid could be incorporated and metabolized in rat tissues. However, a very low percentage of (12,13-[^3]H) cis-12-octadecenoic acid incorporation into tissue lipids was observed. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Nutrition." |
| Physical Description: | xi, 92 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |