Analysis of the human immune response to infection with Schistosoma mansoni : T cell response to egg antigens which elicit granulomatous hypersensitivity /
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| Other Authors: | , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1992.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | Cellular immunity to soluble egg antigens (SEA) of Schistosomiasis mansoni was analyzed in this study. The cellular immune responses of chronically infected patients, former patients, and a SEA sensitized individual demonstrated a variety of patterns to SEA fractionated by HPLC. When PBMC were tested from these same individuals in the in vitro granuloma assay, all participated in granuloma formation. Lines and T cell clones were then generated from the SEA sensitized individual to give a more detailed analysis. When the T cell clones were tested with the 25 HPLC SEA fractions, a much more restricted response was observed. Clone 1F5 reacted to SEA HPLC fraction 18 and 19 and clone 1A8 responded to fractions 1, 19, and 21. When tested in the in vitro granuloma assay, clone 1F5 produced granulomas whereas clone 1A8 and 4C12 did not. Lymphokine analysis revealed that clone 1F5 produced IFN-g, GM-CSF, and TNF, thus it is a Thl clone and clone 1A8, representing a Th2 clone, produced IL-4, IL-5, GM-CSF, and TNF. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Microbiology." |
| Physical Description: | xi, 153 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |