rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: YAC libraries
    sub title: a user's guide
  name:
    name part: Nelson, David L.
    name part: 1956-
  name:
    name part: Brownstein, Bernard H.
  type of resource: text
  origin info:
    place:
      place term: nyu
    date issued: 1994
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xx, 211 pages : illustrations ; 23 cm.
  table of contents: Vectors and host strains for cloning and modification of yeast artificial chromosomes -- YAC cloning vectors -- Accessory vectors for modification of YACs -- Host strains for cloning and manipulation of YACs -- Yeast spheroplast transformation with large DNA -- Aqueous transformations -- Isolation of high molecular weight yeast DNA in agarose plugs -- Inverse PCR of the ends of YAC inserts -- Inverse PCR for identifying intron/exon boundaries -- Subcloning YAC fragments into a lambda vector -- Transfer of YAC DNA into mammalian cells and transgenic mice -- Mammalian selectable markers -- Fusion of yeast spheroplasts with mammalian cells -- Solutions -- Preparation of yeast spheroplasts -- Fusion protocol -- Transformation with total yeast DNA -- Solutions -- Preparation of high molecular weight yeast DNA by centrifugation through a sucrose gradient -- Lipofectin-mediated DNA transfer -- Transformation with gel-purified YAC DNA -- Preparation of YAC DNA by agarasing a gel slice -- Microinjection into tissue-culture cells -- Lipofection into mammalian cells.
  table of contents: (cont) Concentration with a filter unit (up to 600 kb remains intact) -- Microinjection of oocytes for generation of transgenic mice -- Fluorescent in situ hybridization to metaphase chromosomes with YACs -- Current fluorescent in situ hybridization (FISH) -- Implications of FISH for genome mapping -- FISH using YACs -- YAC breeding to reconstitute large loci -- Materials and methods -- Growth media.
  note: edited by David L. Nelson, Bernard H. Brownstein.
  note: Includes bibliographical references and index.
  subject:
    topic: Yeast fungi
    topic: Biotechnology
  subject:
    topic: Yeast fungi
    topic: Genetic engineering
  subject:
    topic: Chromosomes
    topic: Catalogs
  subject:
    topic: Biotechnology
  subject:
    topic: Chromosomes, Fungal
    topic: catalogs
  subject:
    topic: Genetic Engineering
  subject:
    topic: Yeasts
    topic: genetics
  classification: TP248.27.Y43 Y33 1994
  classification: 589.2/330415/0724
  related item:
    title info:
      title: UWBC biotechnical resource series
  identifier: 0716770148
  identifier: 93013780
  record info:
    record content source: DLC
    record creation date: 930427
    record change date: 20151001051721.0
    record identifier: in00001139467