In vivo and in vitro growth of immature embryos of peach (Prunus persica (L.) Batsch) /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1992.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | In vitro ovule manipulation may improve growth of immature peach embryos. The objectives of this study were to evaluate a) in vivo growth of fruits and seed parts and nutrient contents of the developing peach embryos, b) in ovulo growth and quality of immature embryos (8.6 mm) as influenced by media (MSM or SHM), sucrose levels (6% or 10%, ovule support systems (vermiculite, VS; solid Gelrite, GS; filter paper FPS) and c) in ovulo growth and nutrient contents of small embryos (< 5 mm) as influenced by cultural techniques (intact and perforated ovules, or embryo nurse), ovule support system (VS or semi-solid agar, SSA), media (SHM or Monnier), hormones (1, 1.25, 1.50 mg * I^-1 IAA plus 0.01 mg * I^-1 kinetin), and L-glutamine (none or 400 mg * I^-1). In vivo growth trends among cultivars varied in response to the temperature and fruiting set differences between the years. Higher mean temperature and low fruit set promoted faster embryo growth. Fruit fresh weight, ovule length, and ovule fresh weight showed low and inconsistent correlations with embryo length and embryo dry weight; hence, they could not be used as a nondestructive method for embryo selection in the field. Ion vivo embryos had higher macronutrient concentration that in vitro embryos. However, in vitro embryos showed higher dry matter (24%) as compared to in vivo embryos (12%) which may express a better quality embryos. Embryos from ovule perforation treatments showed lower nutrient concentrations than those from intact ovules, although their growth was higher in length and dry weight. The ovule support system influenced embryo (8.7 mm) growth more than media or sucrose. The VS system promoted two to four-fold higher embryo dry weight than FPS or GS, besides being easier to set up and handle that FPS or GS system. The effect of sucrose varied with the medium used; maximum embryo growth occurred with 10% sucrose (w/v) on either SHM or MSM medium, although SHM at 6% sucrose promoted equal growth as SHM with 10% sucrose. The SHM medium was as effective as Monnier medium on growth of small (< 5 mm) embryos, but their effectiveness depended on the technique and ovule support system used... |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Horticulture." |
| Physical Description: | xiii, 95 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |