Expression and inheritance pattern of two foreign genes in petunia plants transformed using the shoot apex method /
| Main Author: | |
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1991.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | A significant number of petunia (Petunia hybrida Vilm.) plants of a commercial variety were genetically transformed using the shoot apex as explant. A binary construct containing genes coding for neomycin phosphotransferase (NPT II) and β-glucuronidase (GUS) was used. Histological studies performed at early stages of the transformation process confirmed expression of the GUS gene in the meristematic regions of the shoot apex. Expression of the gene could be clearly observed in the tunica and corpus layers of meristems of transformed shoots. Studies at the DNA level showed that most transformed plants had stable incorporation of both genes with identical position and copy number. Expression of both genes was highly variable, with total lack of expression of one gene in a significant number of plants. There was a correlation between lack of expression of the NPT II gene and the methylation status of its promoter. Phenotypic expression in progeny of transformed plants had only one plant showing Mendelian ratios for both genes. Many plants presented these ratios for the individual genes. Methylation studies performed in the progeny of some plants showed that this event could explain some of the expression irregularities in the primary transformants and their progeny. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Plant Physiology." |
| Physical Description: | xii, 92 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |