rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Characterization of genes and gene products encoded by the F plasmid transfer region
    sub title: trbI, traW, traU, trbC, traN, and trbE
  name:
    name part: Maneewannakul, Sumit.
    role:
      role term: creator
  name:
    name part: Ippen-Ihler, Karin
    role:
      role term: degree supervisor.
  name:
    name part: Manson, Michael
    role:
      role term: degree supervisor.
  name:
    name part: Struck, Douglas
    role:
      role term: degree committee member.
  name:
    name part: Wilson, Van
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1990
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii, 165 leaves : illustrations ; 29 cm
  abstract: Genes and gene products encoded by the central part of the F transfer region (between traC and traF) were investigated. The nucleotide sequence of the entire region was determined and all gene products were identified and characterized. Three of the tra cistrons in which amber mutations were available and three previously unidentified (trb) genes were found to be included. These mapped in the order: traC trbI traW traU trbC traN trbE traF. Complementation analyses showed traW to be distal to traC rather than proximal as had been suggested in earlier work. The traW gene was shown to express a 210 amino acid, 23,630 Da protein with a 17-19 amino acid signal sequence. The sequence of traW546 amber mutant DNA contained a C [arrow T change in the Gln codon for amino acid 141. Product analysis in maxicells showed that signal processing was inhibited by ethanol; in its absence, mature TraW (21,618-21,760 Da) was found in periplasmic fractions. Similar experiments showed that the product encoded by traU (330 amino acids; 36,786 Da) and trbC (212 amino acids; 23,433 Da) were also processed to 34,275 Da and 21,226 Da mature proteins that were also located in the periplasm. Mutations affecting these three periplasmic proteins are known to affect both F transfer and piliation properties. The traN product, which encoded a 602 amino acid, 65,714 Da precursor, was also subject to signal processing to a 63,823 Da mature protein. However, TraN, which is involved in stable aggregate formation, was found to be an outer membrane protein. Additional experiments showed that TraN spans the outer membrane and is sensitive to hydrolysis when cells are exposed to protease K. The traN548 amber mutation was found to result from a C [arrow] T transition in the 131st codon (Gln) in the traN sequence. In addition, products encoded by trbI (128 amino acids; 14,132 Da) and trbE (86 amino acids; 9,910 Da) were identified...
  note: Typescript (photocopy).
  note: Vita.
  note: "Major subject: Biology."
  note: Ph. D. Texas A & M University 1990
  note: Includes bibliographical references.
  subject:
    topic: Genetic transcription
  subject:
    topic: Gene repression
  subject:
    topic: Plasmids
    topic: Genetics
  subject:
    topic: Protein engineering
  subject:
    topic: Major biology
  identifier: http://gateway.proquest.com/openurl
  identifier: http://proxy.library.tamu.edu/login
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    location:
      url: http://proxy.library.tamu.edu/login
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    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 910905
    record change date: 20200904152525.0
    record identifier: in00001043023
    language of cataloging:
      language term: eng