Cell culture techniques for crop improvement of Oryza sativa (Rice) /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1990.
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| Subjects: | |
| Online Access: | ProQuest, Abstract Link to OAKTrust copy |
| Abstract: | Embryogenesis and plant regeneration of Texas and international rice varieties (both indica and japonica types) were induced in culture on a regime consisting of the use of abscisic acid (ABA) or benzylaminopurine (BAP) in the preculture media and small (10 mg) callus pieces on the regeneration media. Ten 10 mg callus pieces on regeneration media resulted in a 2- to 10-fold increase in plant regeneration over single 100 mg pieces. Plant regeneration of Texas rice cultivars (Lemont, Rico I, Rexmont and Skybonnet) and Taipei 309 was enhanced by the use of ABA in the preculture media with a 2-fold and 3- to 10-fold increase in plant regeneration with 2.6 mgL^-1 and 26 mgL^-1 ABA in the preculture media, respectively. Regeneration of plants from callus of IR36 and IR64 was not enhanced by ABA but by the use of BAP and tryptophan (Trp) in the preculture media or by 2,4-dichlorophenoxyacetic acid (2,4-D) alone. The preculture media containing BAP and Trp allowed a 5-fold increase in plant regeneration from IR64 and was equal to preculture media containing only 2, 4-D for IR36. Acetylsalicyclic acid (ASA) in the preculture medium caused up to a 50% decline in embryogenic callus growth but provided up to a 9-fold increase in regeneration of Rico I at 100 mgL^-1 ASA in combination with 2 mg L^-1 2,4-D and 26 mg L^-1 ABA. Agrobacterium-mediated transformation of the Texas rice varieties was not successful with the protocols used. The selectable agent kanamycin resulted in a bleaching of plants but had little effect on callus growth. The antibiotic G418 provided complete selection against non-transformed shoot tips and callus at levels of 150 mgL^-1. However, habituation of callus on lower levels of G418 allowed survival of callus on levels of G418 up to 250 mgL^01. The effect of another selectable agent, methotrexate, was investigated and found to completely inhibit callus formation at levels of 2 mgL^-1. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Plant physiology." |
| Physical Description: | xiii, 105 leaves ; 29 cm |
| Bibliography: | Includes bibliographical references. |