rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Induction of in vitro capacitation and acrosome reaction in equine spermatozoa
  name:
    name part: Arns, Mark Joseph
    name part: 1959-
    role:
      role term: creator
  name:
    name part: Evans, J. W.
    role:
      role term: degree supervisor.
  name:
    name part: Johnson, L.
    role:
      role term: degree committee member.
  name:
    name part: Martin, J. E.
    role:
      role term: degree committee member.
  name:
    name part: Welsh, T. H.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1989
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xix, 144 leaves : illustrations ; 29 cm
  abstract: Experiments were conducted to investigate the efficacy of heparin, lysophosphatidylcholine (LPC), follicular fluid (FF), cumulus cells (CC) and zonae pellucidae (ZP) to induce in vitro capacitation and acrosome reaction in equine spermatozoa. Spermatozoa cultured with increasing concentrations of heparin did not differ in spontaneous (13%, all treatments) or LPC-induced acrosome reactions (26, 22, 26 & 23 in 0, 10, 50 & 100 ug heparin, respectively). The percentage of acrosome-reacted spermatozoa increased (P <.05) in a concentration- and time-dependent manner after treatment with LPC. Acrosome-reacted spermatozoa were maximal (P <.05) in number after 3.5 h (19, 26, 29, 38 & 49% in 0, 50, 100, 150 & 200 ug LPC treatments, respectively). Fetal calf serum (10%, FCS), 12.5, 25 and 50% FF did not differ in ability to support spontaneous (27, 20, 13 & 26%, respectively) or LPC-induced acrosome reactions (45, 36, 22 & 30%, respectively) after 5 h of culture. Spermatozoa incubated in the absence of protein had higher (P <.05) percentages of spontaneous (49%) and LPC-induced (57%) acrosome reactions. The percentage of viable spermatozoa after 5 h was less (P <.05) in diluents lacking protein (31%) and containing 50% FF (28%) as compared to spermatozoa in diluents containing FCS, 12.5 and 25% FF (55, 62 & 63%, respectively). Inclusion of CC in diluent and subsequent LPC challenge resulted in the highest (P <.05) percentage of acrosome-reacted spermatozoa (24%) following 4 h of culture as compared to spermatozoa unchallenged with LPC either in the absence (17%) or presence (13%) of CC. Spermatozoa challenged with LPC in the absence of CC did not result in a significant (P >.05) increase in the percentage of acrosome-reacted spermatozoa (20%). Spermatozoa challenged with heat-solubilized ZP (1 ZP/ul) had higher (P <.05) incidences of acrosome reactions (27%) as compared to unchallenged spermatozoa (17%) or LPC challenged spermatozoa (22%). In summary, these results indicate th…
  note: Typescript (photocopy).
  note: Vita.
  note: "Major subject: Physiology of reproduction."
  note: Ph. D. Texas A & M University 1989
  note: Includes bibliographical references.
  subject:
    topic: Horses
    topic: Spermatozoa
  subject:
    topic: Horses
    topic: Embryos
    topic: Transplantation
  subject:
    topic: Fertilization in vitro
  subject:
    topic: Major physiology of reproduction
  identifier: http://gateway.proquest.com/openurl
  identifier: http://hdl.handle.net/1969.1/Dissertations-1117101
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://hdl.handle.net/1969.1/Dissertations-1117101
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 910115
    record change date: 20190325092444.0
    record identifier: in00000998235
    language of cataloging:
      language term: eng