Factors influencing the developmental competence of murine chimeras produced by blastocyst reconstitution and multiple embryo aggregation /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1989.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | Although viable offspring have been produced in mice by blastocyst reconstruction and multiple embryo aggregation techniques, previous studies have reported that the developmental competence of the chimeric conceptuses is low. An objective of this investigation was to examine factors that may influence the viability of these chimeras such as: 1) differential rates of development among aggregated embryos according to preimplantation embryonic development (Ped) gene phenotypes, 2) length of exposure to in vitro culture and 3) allogenic incompatibilities between the maternal host and chimeric conceptuses. An additional objective was to evaluate the use of giant trophectodermal (TE) vesicles as a strategy enhancing the viability of murine reconstituted blastocysts. Embryos (8-12 cell) were recovered at 2.8 days post-coitum (p.c.) from hybrid, parental backcross (B6D2-F1xBL/6), inbred (C3H x C3H) and randomly-bred (ICR x ICR) murine genotypes with experimentally verified fast, slow and mixed Ped gene phenotypes, respectively. Zone-free embryos were cultured in modified Whitten's medium as singletons or aggregates containing 4-5 embryos each. After 0.7 or 1.7 days in vitro culture, embryo singletons or aggregates were transferred to allogenic or synergenic recipients. Nonmanipulated control embryos were collected at 3.5 days p.c. and immediately transferred to ICR recipients.. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Veterinary physiology." |
| Physical Description: | ix, 114 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |