Investigation of the in vitro incorporation of exogenous fatty acids into bovine intramuscular and subcutaneous adipose tissues /
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| Other Authors: | , , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1989.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Abstract: | Twelve Angus cattle (6 steers and 6 heifers) of the same age groups finished with a standard finishing diet were used to study the exogenous fatty acid esterification activities of intramuscular and subcutaneous adipose tissues in vitro. Of the cattle, 3 steers and 3 heifers were fasted for 72 hr prior to slaughter whereas the remainder was slaughtered 4 hr after removal from feed. At slaughter, intramuscular and subcutaneous adipose tissues were obtained from the 8-13th rib region of longissimus dorsi muscle for cellularity determinations and in vitro assays. Adipose tissue snips were incubated with the radioisotopically labeled fatty acid (palmitate, stearate, oleate, linoleate or linolenate) at three different concentrations (0.3 mM, 0.6 mM and 2.0 mM) to assess the rates of fatty acid incorporation into glycerolipids. Using adipose tissue homogenates as enzyme sources, rates of glycerol 3-phospiiate esterification were determined in the presence of [U-14C]L-glycerol 3-phosphate and fatty acids, while the release of inorganic phosphate from aqueous dispersed phosphatidate was taken as a measure of phosphatidate phosphohydrolase activity. The rates of fatty acid uptake and esterification in adipose tissues increased with fatty acid concentration in vitro. For all the fatty acids investigated, triglycerides were the predominant products (60-85%), and diglycerides were the subsidiary (9-20%) lipid class. No apparent rate limiting step in triglyceride synthesis was noted in the tissue incubations. Intramuscular and subcutaneous adipose tissues incorporated the five different fatty acids into tissue lipids at dissimilar rates which were: palmitate > oleate > linolenate > linoleate > stearate.. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Animal Science." |
| Physical Description: | xvi, 174 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |