Characterization of heterotrophic and photomixotrophic growth of callus and suspension cultures of Pinus elliottii and Glycine max /

Bibliographic Details

Main Author:	Treat, William Jay, 1955-
Other Authors:	Baldwin, Thomas O. (degree committee member.), Stout, Bill A. (degree committee member.), Sweat, Vince E. (degree committee member.)
Format:	Thesis Book
Language:	English
Published:	1988.
Subjects:	Bioreactors. Callus (Botany) Plant cell culture. Slash pine. Soybean. Photomixotrophic callus and suspension cultures of P. elliottii were established. The selective use of environmental pressures such as increased C 02 and decreased O2 in the inlet gas, a decrease in the medium sucrose and macro-nutrients, and an increased photoperiod to 24 h helped to develop these cell lines. Electron micrographs showed that secondary metabolites and starch could exist in P. elliottii callus cell simultaneously. No secondary metabolites were observed in the HT callus, HT suspension, and PMT suspension. These micrographs served to document the physical structure of the cells at the time of subculture. Furthermore, inferences can be draw about the physical structures and the tissues physiological state. Interest in bioreactor technology has been renewed with the advent of large scale suspension cultures of higher plant and animal cells. The bioreactor with all its control mechanisms offers a significant research tool to study the physiology of phototrophic cells that cannot be conducted in shake flasks. Results of this study have demonstrated that a stirred tank reactor equipped with a cell lift impeller can be used to effectively culture photomixotrophic P. elliottii and Glycine max suspensions. Academic theses.
Online Access:	Link to ProQuest copy Link to OAKTrust copy ProQuest, Abstract