Effects of petroleum creosote on selected stages of embryonic development /
| Main Author: | |
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1989.
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| Subjects: | |
| Online Access: | ProQuest, Abstract Link to OAKTrust copy |
| Abstract: | The prenatal toxicity of petroleum creosote, a complex mixture of chemicals, was investigated via an in vivo study and an in vitro embryo culture system. Additionally, the prenatal toxicity of naphthalene, one chemical component of petroleum creosote, was determined in the in vitro system. The purpose of the study was to provide specific data on the prenatal toxicity of petroleum creosote and demonstrate the value of the two techniques. In the in vivo study, petroleum creosote was not embryotoxic or teratogenic in ICR mice when administered on gestation days 5-9, at a dose of 400 mg/kg body weight. In vitro, petroleum creosote becomes embryotoxic to ICR mouse blastocysts at some exposure level between 22 and 33 μg/ml of media. Bioactivation plays a major role in embryotoxicity of naphthalene. Naphthalene without rodent liver microsomal enzymes added to the media was not embryotoxic at levels as high as 100 μg/ml media, whereas naphthalene became embryotoxic at some level between 10 and 50 μg/ml of media in the presence of microsomes. The data indicate that naphthalene is one of the embryotoxic components of petroleum creosote, and that exposure to sufficient levels of petroleum creosote during early pregnancy could result in embryonic loss. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Veterinary Anatomy." |
| Physical Description: | xi, 83 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |