Oxytocin-induced prostaglandin release from uterine tissues : in vivo and in vitro studies /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1989.
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| Subjects: | |
| Online Access: | Link to ProQuest copy Link to OAKTrust copy ProQuest, Abstract |
| Abstract: | Three studies were designed to determine: 1) if the postpartum uterus responds to oxytocin (OT) by releasing prostaglandins (PG) in a dose dependent manner; 2) compare the ability of caruncular (C) and intercaruncular (IC) endometrial tissue types to release PG in response to OT on day 20, 30 or at first estrus postpartum; and 3) determine if pregnancy-specific protein B (PSPB) can modify PG release by bovine uterine endometrial tissue on day 16 of the estrous cycle. In Experiment I, the previously gravid uterine horn was surgically removed on either day 20 (n=7) or day 30 (n=7) whereas the uterine horn ipsilateral to the dominant follicle was removed 12-18 h after the onset of first behavioral estrus postpartum (E; n=8). Tissues (IC and C; 200 mg wet wt) were cultured in Nutrient Mixture F-10 media in a perfusion system. Media and tissues were saturated with 95% O2: 5% CO2, temperature was 39°C, flow rate was 100 μ/min and fractions were collected at 20 min intervals. Tissues were challenged with 1, 2 or 4 μg [Asu^1.6]-OT for 1 h and perifusion continued for 280 min. All doses of OT elevated prostaglandin F[2α] (PGF) and prostaglandin E2 (PGE) release (P <.0001) tissue (P <.0001) and day x tissue (P <.03) affected PGF release. Release of PGF in response to OT was higher on day 20 than day 30 with day 30 higher than E (P <.007). Intercaruncular tissue released more PGF than C (P <.0001). Release of PGE in response to OT was higher on days 20 and 30 than E (P <.008). Basal PGE and PGF were greater on day 20 than 30 or E (P <.02). In Experiment II, eighteen Brahman cows were given either 30, 150 or 300 IU of OT i.v. on days 10, 20 and 30 postpartum. Day, time and day x dose affected plasma 13, 14-dihydro-15-keto prostaglandin F[2α] (PGFM) concentration (P <.0001). All does of OT raised plasma PGFM on all days (P <.0001), difference between doses were not detected... |
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| Item Description: | "Major subject: Physiology of Reproduction." Typescript (photocopy). Vita. |
| Physical Description: | xii, 99 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |