Metabolic and cytological changes in calli, seedlings and whole plants of Sorghum bicolor(L.) Moench under induced salt and water stress /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
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1988.
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| Online Access: | Link to OAKTrust copy |
| Abstract: | Research was conducted to study the biochemistry and physiology of sorghum cells under imposed water stress. Specifically, rapid changes in de novo protein synthesis in callus cultures of two sorghum cultivars, one showing drought tolerance and one showing drought intolerance under field conditions, were monitored using mannitol and NaCI as osmotic agents. The changes in protein patterns were assessed qualitatively by examination of protein bands obtained after one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and quantitatively by densitometric analysis of fluorographs. The callus of the "intolerant" (Dl) cultivar (BTx 3197) showed more changes in the pattern of newly synthesized proteins under mannitol-induced water stress conditions than the "tolerant" (DT) cultivar (RTx 430). No major changes in protein patterns were observed in the NaCI treatments. Studies with leaf tissue of whole plants revealed that a protein of molecular weight 39 kD was synthesized in both cultivars under long-term stress conditions but not under control conditions. This protein was not observed in callus cultures nor in seedlings grown for prolonged periods in mannitol- and NaCI-supplemented media. The activity of arginine decarboxylase (ADC), a stress-induced enzyme, was monitored in callus cultures, seedlings and leaf tissue from the whole plants under control and water stress conditions. In the stress treatments of the DT cv, the enzyme showed activities equal or higher to the control in callus and leaf tissue, respectively. In contrast, stress treatments of the Dl cv showed levels of ADC well below control levels in both callus cultures and in leaf tissue. These results suggest that ADC could be playing a role in stress tolerance. Morphological changes in sorghum cultures grown in mannitol- and NaCI-supplemented media were observed. After prolonged mannitol and NaCI stress, cells from the Dl cv were reduced in size whereas the cells from the DT cv showed increased size. The data suggest that the cells from the DT cv are capable of undergoing osmotic adjustment under salt and mannitol stress. |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Plant Physiology." |
| Physical Description: | xiii, 87 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references. |