rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Characterization of the antibody response to Brucella abortus S2308 challenge infection in cattle
  name:
    name part: Estes, Don Mark.
    role:
      role term: creator
  name:
    name part: Adams, L. Garry
    role:
      role term: degree committee member.
  name:
    name part: Smith, James D.
    role:
      role term: degree committee member.
  name:
    name part: Smith, Roger
    role:
      role term: degree committee member.
  name:
    name part: Templeton, Joe W.
    role:
      role term: degree supervisor.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1988
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 69 leaves : illustrations ; 29 cm
  abstract: Attempts to define the antibody response to Brucella abortus vaccination and challenge have been contradictory in assessing the relative importance of specific isotypes and their expression in resistant and susceptible cattle. We approached the resolution of the antibody response to Brucella abortus at both the population level in immunized cattle and in a group of nonimmunized naturally resistant and susceptible cattle. The lack of reagents reactive with newly recognized IgG subisotypes necessitated production of subisotype-specific monoclonal antibodies in addition to allotype-specific reagents. The antibody response to Brucella abortus infection in immunized and nonimmunized cattle was assessed by solid-phase enzyme-linked immunosorbent assay (ELISA). The responsiveness in resistant and susceptible cattle was determined using B. abortus low (2%) protein lipopolysaccharide (LPS) and whole cells of the rough "O" antigen deficient strain, RB51. Irrespective of immunization status, the postchallenge lgG1 RB51-specific and LPS-specific responses were of greatest value in predicting the outcome of infection. Immunized animals received either rough cell envelopes or 2% protein LPS in RIBI adjuvant or were treated with the live vaccine strain of B. abortus (S19). LPS-specific post-immunization levels were highest for the lgG1 and lgG2a subisotypes. The lgG2b total levels postchallenge differentiated culture negative and culture positive cattle. Immunoglobulin allotype expression was evaluated by the LPS or RB51 whole cell ELISA in 24 nonvaccinated cattle challenge exposed with B. abortus 2308. The Brucella antigen-specific ELISA indicated persistent levels of LPS-specific lgG2a in nonimmunized susceptible cattle as long as 36-48 months postchallenge while nonimmunized resistant animals had >10 fold lower lgG2a levels relative to pre-exposure levels. The skewed expression of lgG2a(A1) in immunized culture positive cows and nonimmunized susceptible cows in the Brucella ant…
  note: "Major subject: Genetics."
  note: Typescript (photocopy).
  note: Vita.
  note: Ph. D. Texas A & M University 1988
  note: Includes bibliographical references (leaves 63-68).
  subject:
    topic: Brucella abortus
  subject:
    topic: Brucellosis in cattle
  subject:
    topic: Immunospecificity
  subject:
    topic: Major genetics
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-803429
  identifier: http://gateway.proquest.com/openurl
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-803429
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 891205
    record change date: 20220104090429.0
    record identifier: in00000790436
    language of cataloging:
      language term: eng