rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Analysis of the cis-acting transcriptional regulatory sequences associated with mouse mammary tumor virus proviral DNA
  name:
    name part: Toohey, Mark Gerard
    name part: 1956-
    role:
      role term: creator
  name:
    name part: Lohman, Timothy M.
    role:
      role term: degree committee member.
  name:
    name part: Mullet, John E.
    role:
      role term: degree committee member.
  name:
    name part: Peterson, David O.
    role:
      role term: degree supervisor.
  name:
    name part: Thomas, Terry L.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1988
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 116 leaves : illustrations ; 29 cm
  abstract: The transcriptional activity of the mouse mammary tumor virus (MMTV) promoter is positively regulated by the action of glucocorticoid hormones. This action is thought to be mediated, at least in part, by the binding of the glucocorticoid hormone receptor complex (HRC) to specific DNA sequences, collectively called the glucocorticoid responsive enhancer element (GRE), located within each of the proviral long terminal repeats (LTRs). Other sequences within the proviral genome have also been shown to specifically bind the HRC in vitro, but their function as GREs has not been tested in a defined sequence context. These observtions suggest the possibility that the overall levels of glucocorticoid hormone-mediated transcription from the MMTV promoter may be modulated by the number of GREs associated in cis with this promoter. This possibility was tested utilizing transient transfection experiments, in which promoter activity was monitored either by way of expression of the bacterial chloramphenicol acetyltransferase gene or by direct analysis of RNA transcripts. The results indicate that the overall levels of transcription from the MMTV promoter is modulated in an additive way by the number of functional GREs derived from the MMTV LTR. Other sequences within the MMTV genome which bind the glucocorticoid hormone receptor complex in vitro, were not able to mediate such additive effects. In addition, neither these, nor any other sequences from within the coding region of the provirus, were functional when directly tested for their ability to serve as GREs, indicating that they do not act in such a manner in the intact MMTV provirus. However, sequences located on a 1.8 kilobase DNA fragment from within the proviral genome, were able to act as a constitutive enhancer element when linked to a region of the MMTV LTR which contains the MMTV promoter. RNA analysis indicate that this constitutive enhancer element may affect a previously unrecognized promoter within the MMTV LTR. Th…
  note: "Major subject: Biochemistry."
  note: Typescript (photocopy).
  note: Vita.
  note: Ph. D. Texas A & M University 1988
  note: Includes bibliographical references.
  subject:
    topic: Genetic regulation
  subject:
    topic: Glucocorticoids
  subject:
    topic: Major biochemistry
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-783215
  identifier: http://gateway.proquest.com/openurl
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-783215
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 890719
    record change date: 20220104090409.0
    record identifier: in00000769564
    language of cataloging:
      language term: eng