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mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Investigations into the replication, structure, and expression of the Campoletis sonorensis virus genome
  name:
    name part: Theilmann, David Allan
    name part: 1958-
    role:
      role term: creator
  name:
    name part: Summers, Max D.
    role:
      role term: degree supervisor.
  name:
    name part: Thomas, Terry L.
    role:
      role term: degree supervisor.
  name:
    name part: Peterson, David O.
    role:
      role term: degree committee member.
  name:
    name part: Sauer, Helmut W.
    role:
      role term: degree committee member.
  name:
    name part: Vinson, S. Bradleigh
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1988
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: x, 130 leaves : illustrations ; 29 cm
  abstract: The polydnavirus Campoletis sonorensis Virus (CsV) is known to infect m any tissues of the parasitized host larvae Heliothis virescens. Even though expression of CsV is known to occur in H. virescens tissue no replication has been observed. In this study CsV DNA was analyzed in H. virescens tissue using sensitive molecular hybridization techniques. The results showed that in naturally parasitized or calyx fluid injected H. virescens larvae, no increase in the level of viral DNA could be detected for the entire period of endoparasite development. Replication could only be detected in late pupal and adult stages of C. sonorensis wasps. These results suggest that CsV does not replicate in H. virescens larvae and may be restricted to the tissues of C. sonorensis which has novel implications with respect to the life cycle of this virus. To obtain defined probes for analysis of viral transcription superhelical (SH) CsV DNAs were cloned and partially physically characterized. Five SH clones from the B, H, M, O^1 and W regions of the CsV genome were analyzed for sequence relationships with respect to the rest of the CsV genome. All of the DNAs were found to cross-hybridize to multiple regions in the CsV genome. In addition, there was an increase in the number of regions to which cross-hybridization was observed if the stringency of hybridization was reduced. On SH-B, H, and O^1 an imperfectly conserved repeat sequence was identified and sequenced and was shown to have an average size of 540 bp. The repeat elements were arranged singly or in direct tandem arrays. These results show that the CsV genome is comprised of interrelated SH DNAs that may have arisen by multiple recombinational events and sequence divergence. Each of the cloned SH DNAs were used to analyze viral transcription in the C. sonorensis female reproductive tracts and it was shown that CsV is expressed in the adult female w asp. This is the first report to identify viral transcripts in the w asp host. Compar…
  note: Typescript (photocopy).
  note: Vita.
  note: "Major subject: Microbiology."
  note: Ph. D. Texas A & M University 1988
  note: Includes bibliographical references (leaves 113-127).
  subject:
    topic: DNA viruses
  subject:
    topic: Host-virus relationships
  subject:
    topic: Insects
    topic: Viruses
  subject:
    topic: Viral genetics
  subject:
    topic: Major microbiology
  identifier: http://gateway.proquest.com/openurl
  identifier: http://hdl.handle.net/1969.1/Dissertations-783040
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://hdl.handle.net/1969.1/Dissertations-783040
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 890712
    record change date: 20190325092236.0
    record identifier: in00000769390
    language of cataloging:
      language term: eng