Isolation and partial characterization of arachin and [alpha]-conarachin from the seeds of Arachis hypogea L. /
| Main Author: | |
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| Other Authors: | , , , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
[College Station, Tex.] :
Yu,
1977.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy Link to ProQuest copy |
| Abstract: | Peanut, a major food crop, is particularly rich in protein which constitutes about one-fourth of its dry w eight. The investigation of the biochemical nature of this important seed's proteins is the author's dissertation project so that the protein structures and com position might be better understood. Starting from the raw peanut meat, defatted meal was prepared and the proteins were extracted with different buffers, such as 10% NaCl, phosphate and tris citrate buffer at different ionic strengths. Arachin was prepared according to the methods previously reported, such as ammonium sulfate fractionation, cryoprecipitation and precipitation of arachin by calcium chloride. Other method such as salting-out by potassium phosphate fractionation was also used. Similarly, conarachin was also prepared by ammonium sulfate fractionation and by removal of arachin through cryoprecipitation and calcium chloride precipitation. Crude arachin and conarachin were chromatographed on Sephadex, DEAE-cellulose and hydroxylapatite columns for further purification. Three comprehensive isolation schemes were used to prepare arachin AM45PPSP and AM85PS, conarachin YD and arachin ETCPPP. Purification of SDS-denatured total peanut protein, arachin and a-conarachin was carried out on Sephadex and controlled pore glass columns.. |
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| Item Description: | Vita. "Major subject: Biochemistry." |
| Physical Description: | xvi, 224 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references (leaves 220-223). |