Californium-252 plasma desorption mass analysis of proteins adsorbed on polymer and modified-polymer surfaces.

Bibliographic Details
Main Author:	Hill, John Chester
Other Authors:	Naugle, D. G. (degree committee member.), Russell, D. H. (degree committee member.), Schweikert, E. A. (degree committee member.)
Format:	Thesis Book
Language:	English
Published:	1987.
Subjects:	Plasma desorption mass spectrometry. Proteins > Absorption and adsorption. Major chemistry. Academic theses
Online Access:	Link to ProQuest copy Link to OAKTrust copy

Description
Abstract:	A new Cf-252 plasma desorption mass spectrometer has been built specifically for the analysis of large biomolecules. This mass spectrometer was used to investigate the interactions between proteins adsorbed onto polymer surfaces and how the chemical nature of the polymer surface influences the production of stable, gas-phase molecular ions. Chemical modification of the polymer surfaces was achieved by means of ultra-violet irradiation, resulting in the production of a more hydrophilic surface. Analysis of a series of model compounds adsorbed onto modified and non-modified polymer surfaces indicates that the wettability of the surface is an important influence in the production of stable molecular ions. This information was then utilized to aid in the analysis of lysozyme, myoglobin, and porcine trypsin.
Item Description:	Typescript (photocopy). Vita.
Physical Description:	xiv, 243 leaves : illustrations ; 29 cm
Bibliography:	Includes bibliographical references (leaves 226-233).