An in vitro and in vivo study of the pathogenesis of Mouse Hepatitis Virus-4 infection /
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1980.
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| Subjects: | |
| Online Access: | ProQuest, Abstract Link to OAKTrust copy |
| Abstract: | The pathogenesis of Mouse Hepatitis Virus-4 (MHV-4) infection was studied in primary confluent murine astrocytic monolayers, murine cerebellar organotypic explants (both during and after the initial period of rapid myelination in vitro), and in the intact weanling mouse. Varying inoculum concentrations of tissue culture adapted virus were used in each of the in vitro studies. Astrocytic monolayers inoculated with a high concentration of virus developed a severe necrotizing cytopathic effect (CPE). A lower concentration of virus resulted in the formation of astrocytic syncytia, cytoplasmic inclusion bodies, and a steady-state infection in which direct cell-to-cell transmission of virus was detected. Immature cerebellar explants inoculated with a high concentration of virus before myelination occurred in vitro developed a necrotizing CPE characterized by necrosis of granule cells and the production of edema. Syncytia and cytoplasmic inclusion bodies also developed within the astrocytic outgrowth. Direct cell-to-cell transmission of virus was detected. Demyelination was located mainly in focal areas of necrosis which were also edematous. Surviving axons were myelinated, or were being myelinated, by 14 days post-inoculation (DPI). Oligodendroglia infrequently had degenerative changed. The low dosage of virus resulted in a milder CPE with the formation of syncytia and inclusion bodies in the astrocytic outgrowth and focal myelin destruction in areas of necrosis. Mature myelinated explants inoculated with a high dosage of virus developed an acute necrotizing CPE which involved all areas of infected explants. The intermediate and low dosages of virus resulted in a CPE which most severely affected the non-neuronal (white matter) areas and was characterized by edema, hydropic degeneration of infected astrocytes, and in explants inoculated with the low viral dosage, primary demyelination and persistent infection in which direct cell-to-cell transmission of virus was detected. Demyelination with the low viral dosage was concluded to be associated with edema which was largely due to the hydropic degeneration of infected astrocytes... |
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| Item Description: | Typescript (photocopy). Vita. "Major subject: Veterinary Pathology." |
| Physical Description: | xvi, 168 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references (leaves 161-167). |