rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Protein crystallography
    sub title: new approaches to X-ray data collection, direct space refinement and model building
  name:
    name part: Legg, Margaret Judith.
    role:
      role term: creator
  name:
    name part: Cotton, F. A.
    role:
      role term: degree supervisor.
  name:
    name part: Glover, G. I.
    role:
      role term: degree committee member.
  name:
    name part: Hazen, E. E.
    role:
      role term: degree committee member.
  name:
    name part: Pace, C. N.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: txu
    date issued: 1977
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: x, 111 leaves : illustrations ; 28 cm
  abstract: This crystallographic study was designed to develop and test efficient techniques of X-ray data collection, phase extension and refinement, and model building capable of giving a reliable description of macromolecules in particular the extracellular nuclease of Staphylococcus aureus. Since protein crystals are irreparably damaged upon exposure to X-radiation, the data collection procedure minimized radiation damage by measuring first only the raw peak intensities using limited oi step scans and by sampling the background levels subsequent to all peak intensities. In testing the limited three step w scanning technique on a small molecular complex, the w scanning procedure gave the same refined model as the standard 0/2θ scanning method. In order that the peak intensity data could be measured with a minimal amount of radiation damage, a background collection procedure was developed which would sample the backgrounds of the entire range of data subsequent to peak data collection. Using Fourier interpolation and smoothing techniques, the sampled background was manipulated to give reliable estimates of the individual backgrounds. The combination of these two procedures allowed the collection of a complete 1.5A data set for the pdTp inhibited form of nuclease. To obtain higher resolution maps than that afforded by isomorphous replacement techniques, a direct space refinement procedure developed by Collins (1975) was tested on the protein rubredoxin and was used to refine and extend the phasing for nuclease. In both cases the direct space refined map was significantly improved over the initial map phased by isomorphous replacement methods..
  note: by Margaret Judith Legg.
  note: "Major subject: Chemistry."
  note: Vita.
  note: Texas A & M University
  note: Includes bibliographical references (leaves 96-99).
  subject:
    topic: Proteins
    topic: Research
  subject:
    topic: X-ray crystallography
  subject:
    topic: Chemistry
  subject:
    topic: Proteins
    topic: Research
  subject:
    topic: X-ray crystallography
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-620695
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-620695
  record info:
    record content source: TXA
    record creation date: 780621
    record change date: 20220104090157.0
    record identifier: in00000595208
    language of cataloging:
      language term: eng