rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Biochemical and genetic studies of wild type and attenuated vaccine strains of bluetongue virus
  name:
    name part: Samal, Siba Kumar
    name part: 1955-
    role:
      role term: creator
  name:
    name part: Estes, Mary K.
    role:
      role term: degree committee member.
  name:
    name part: McConnell, Stewart
    role:
      role term: degree supervisor.
  name:
    name part: Ramig, Robert F.
    role:
      role term: degree supervisor.
  name:
    name part: Tizard, Ian R.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1985
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiii, 207 leaves : illustrations ; 29 cm
  abstract: A biochemical, and genetic examination of the tetravalent, attenuated vaccine for bluetongue virus (BTV) developed at Texas A&M was undertaken. These studies were aimed at (i) identifying reliable vaccine-associated markers, (ii) determining the genetic stability of the vaccine, and (iii) examining the molecular basis of attenuation in BTV. Comparative growth studies showed that maximal yields of all strains were obtained when cell monolayers were harvested early after the onset of cytopathic effects. One-step growth curves showed an altered growth kinetics for type 13 V. The four V strains were temperature-sensitive (ts) for growth at 39° and for plaque formation at 37°. Recombination tests indicated that the ts lesions of the vaccine strains were on different reassorting genome segments. Electrophoretic comparisons of the genome RNAs, proteins, and protease digests of individual proteins species from homotypic W:V pairs revealed numerous differences. Only changes in segment 2 and its product were seen in all four W:V pairs. Changes in genome segment 2 of 10V, 11V, and 13V were detected by both direct electrophoretic analysis of genomic RNAs and partial protease analyses of the segment 2 gene product. Changes in genome segment 2 of 17V could only be inferred from the heat lability of the segment 2 product. Analyses of mixed infections between heterotypic BTV strains revealed that reassortment of genome segments between V strains and W strains, occurred at high frequency. These analyses also allowed the tentative assignment of the ts lesions of 10V and 17V to genome segments 4 and 5, respectively. This study has yielded significant results relative to the original aims. Several differences in the biological and biochemical properties of the vaccine strains that may serve as reliable vaccine markers were identified. The results of this study have been used to construct the hypothesis that mutations in genome segment 2 of the vaccine strains are responsible for the at…
  note: "Major subject: Veterinary Microbiology."
  note: Typescript (photocopy).
  note: Vita.
  note: Ph. D. Texas A & M University 1985
  note: Includes bibliographical references (leaves 188-206).
  subject:
    topic: Arboviruses
  subject:
    topic: Bluetongue virus
    topic: Genetic aspects
  subject:
    topic: Cattle
    topic: Diseases
    topic: Prevention
  subject:
    topic: Major veterinary microbiology
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-450230
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-450230
  record info:
    record content source: TXA
    record creation date: 870504
    record change date: 20220103135022.0
    record identifier: in00000443897
    language of cataloging:
      language term: eng