Biological characteristics of avian Chlamydia psittaci.
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
1984.
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| Subjects: | |
| Online Access: | Link in ProQuest Copy Link to OAKTrust copy |
| Abstract: | Seventeen isolates of Chlamydia psittaci from several avian species were examined to determine: (1) their growth characteristics in L-929 cells; (2) the effect of host cell treatment with various chemical agents on their infectivity; (3) whether or not the isolates could be grouped according to their responses to the various host cell treatments; (4) their protein profiles; (5) antigenic relationships between the isolates; and (6) the relationships of their major outer membrane proteins. These isolates were divisible into 2 major groups based on their infectivity for untreated cells. The treatment of cells with DEAE-dextran plus cycloheximide or 5-iodo-2-deoxyuridine (IUDR) increased the infection rate of 8 isolates. DEAE-dextran significantly increased the infection rate of 3 isolates while cycloheximide and Vibrio cholerae neuraminidase increased the infection rate of 7 isolates. Cycloheximide, DEAE-dextran plus cycloheximide, IUDR, and neuraminidase each significantly decreased the infection rate of 1 isolate. Wheat germ agglutinin had no significant effect on the infection rate of any isolate. While infectivity for untreated cells appears to be a good character to separate these isolates. Differences in the major outer membrane proteins (MOMP) of the isolates were ascertained. These differences corresponded to the differences in infectivity of these isolates. Those which were most infective for L-929 cells had a MOMP molecular weight of 43.5 kilodaltons (kd). The low infectivity group had an MOMP molecular weight of 45.5 kd. The avian isolates of C. psittaci had some high molecular weight proteins associated with their outer membranes. Immunoblots were used to determine antigenic relationships between the isolates. A 29 kd protein reacted only with antisera raised against avian isolates of C. psittaci. The high molecular weight proteins that were associated with MOMP of C. psittaci reacted only with antisera against the avian isolates but not with antiserum against C. trachomatis. Reactions with the MOMP's differentiated between the high and low infectivity isolates because of specificity of the respective antisera. Reactions with a 49 kd protein were not necessarily species specific but did indicate relationships of the low infectivity group isolates of avian C. psittaci with C. trachomatis. |
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| Item Description: | "Major subject: Veterinary Microbiology." Typescript (photocopy). Vita. |
| Physical Description: | ix, 80 leaves : illustrations ; 29 cm |
| Bibliography: | Includes bibliographical references (leaves 73-79). |