Enzymatic modification of cottonseed proteins /
| Main Author: | |
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
[College Station, Tex.] :
Cunningham,
1977.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy Link to ProQuest copy |
| Abstract: | Work with cottonseed proteins was undertaken to develop methods of enzymatic modification and protein recovery that might have use in increasing the utilization of cottonseed proteins as a source of human nutrition. Protein modification by the use of enzymes present within the seed was evaluated by germination of resting cottonseed. Germination was investigated as a potential process for treatment of cottonseed prior to protein extraction. As the germination process proceeds, protein solubility increases. The electrophoretic mobility of cottonseed protein changed with germination time but the ultracentrifuge patterns of cottonseed proteins germinated through 4 days did not change. Germination produced significant changes in the amino acid composition of the seed but had little effect on the composition with respect to the essential amino acids. In addition to the use of the enzymes present within germinating cottonseed, other non-cottonseed enzymes were used for protein modification. Standardized procedures for direct comparison of enzyme performance were investigated. In all, 11 different enzymes were applied to various cottonseed protein products to determine their effect on protein solubility. Numerous enzymes and treatments were presented and ail enzymes were able to increase protein solubility. Since it was determined that numerous enzymes are capable of increasing the protein solubility of cottonseed proteins, recovery methods for the soluble proteins were evaluated by incorporation of the modifying enzyme into an ultrafiltration device, resulting in immobilization of the enzyme. An ultrafiltration ceil and a hollow-fiber device were evaluated for their potential in production of enzymatically modified proteins. Both systems were operable in a continuous mode but at the same time both systems had low resolution in terms of the ability of the membrane to provide efficient separation of one size molecule from another.. |
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| Item Description: | Vita. "Major subject: Biochemistry." |
| Physical Description: | xiii, 126 leaves : illustrations ; 28 cm |
| Bibliography: | Includes bibliographical references (leaves 121-125). |