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mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Biochemical characterization of chromatin from the dinoflagellate Crypthecodinium cohnii, the binucleate dinoflagellate Peridinium balticum and a chromophyte Olisthodiscus luteus
  name:
    name part: Shupe, Kathleen
    role:
      role term: creator
  name:
    name part: Burghardt, Robert C.
    role:
      role term: degree committee member.
  name:
    name part: Johnson, James R.
    role:
      role term: degree committee member.
  name:
    name part: Rizzo, Peter J.
    role:
      role term: degree supervisor.
  name:
    name part: Wild, James R.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1982
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xiii, 145 leaves : illustrations ; 29 cm
  abstract: Understanding of the complex genetic control mechanisms of higher organisms may be facilitated by examining primitive organisms that possess much lower degrees of gene complexity, yet have developed advanced modes of gene transcription, replication, and organization. The group of organisms collectively called the algae is of particular interest with respect to chromatin structure due to the tentative classification of many of its members. Classification places a few species close to higher plants while others border on the lower plant-protozoan boundary. Chromatin from a uninucleate dinoflagellate, Crypthecodinium cohnii, a binucleate dinoflagellate, Peridinium balticum and a chromophyte, Olisthodiscus luteus, were examined. Scanning electron microscopy reveals recognizable differences between the dinokaryotic nucleus and the eukaryotic nucleus. The eukaryotic nucleus reveals a very smooth surface when viewed with the scanning microscope and nuclear pores are evident. Scanning electron microscopy revealed a dinokaryotic nucleus whose nuclear envelope was highly convoluted. Higher magnification of the extruded chromatin from the dinokaryotic nucleus reveals fibers ranging from 400 to 500 (ANGSTROM). That these fibers are higher order structures of the unit thread does not seem likely. Similar to higher eukaryotes, staphylococcal nuclease digestion of nuclei isolated from O. luteus reached a limit of 50% digestion. In contrast, digested C. cohnii nuclei only reached a 20% limit of digestion. Digestion of nuclei isolated from P. balticum exhibited characteristics expected from a mixture of dinokaryotic and eukaryotic nuclei. Gel analysis of the products of digestion of the eukaryotic nucleus from Olisthodiscus and Peridinium revealed a basic repeat unit measuring 220 (+OR-) 5 base pairs. Limit digestion gave a core particle of 140 base pairs revealing that these longer repeat sizes are due to longer linker regions. No repeating subunit structure was found upon electrop…
  note: "Major subject: Microbiology."
  note: Typescript (photocopy).
  note: Vita.
  note: Ph. D. in Philosophy Texas A & M University 1982
  note: Includes bibliographical references (leaves 133-144).
  subject:
    topic: Algae
    topic: Cytology
  subject:
    topic: Chromophyta
  subject:
    topic: Dinoflagellates
  subject:
    topic: Microbiology
  identifier: http://proxy.library.tamu.edu/login
  identifier: https://hdl.handle.net/1969.1/DISSERTATIONS-350232
  identifier: http://gateway.proquest.com/openurl
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    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: https://hdl.handle.net/1969.1/DISSERTATIONS-350232
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 830811
    record change date: 20220103135446.0
    record identifier: in00000342242
    language of cataloging:
      language term: eng