Application of a Leydig cell stimulation assay to quantitate circulating levels of luteinizing hormone in the bovine, baboon and equine /
| Main Author: | |
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| Other Authors: | , , |
| Format: | Thesis Book |
| Language: | English |
| Published: |
[College Station, Tex.] :
French,
1978.
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| Subjects: | |
| Online Access: | Link to ProQuest copy. Link to OAKTrust copy |
| Abstract: | A Leydig cell stimulation assay for luteinizing hormone was applied to the analysis of LH activity in serum samples from the baboon, the bovine, and the equine. Close parallelism was seen between dose response curves for purified bovine LH, hCG, bovine serum, baboon serum, and equine serum. There was good correlation between the level of serum LH in samples assayed by both RIA and LCSA. The pattern of LH secretion around the time of ovulation that was detected by LCSA from samples in this study was in agreement with expected results based on literature reports of LH patterns determined by other sasay systems. Although not conclusive, evidence presented in this study supports the conclusion that LH activity can be measured by LCSA in the serum of intact cows. Data presented indicate that serum LH levels in the cycling mare and baboon can also be measured. The LCSA requires about eight hours to perform, including preparation of the Leydig cell suspension. The testes of one mouse provides enough cells for 150 assays. The sensitivity of the LCSA described here was 3 pg of LER-1072-2. A sensitivity of 600 pg/ml of serum was shown after at least a 1:20 dilution of samples. |
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| Item Description: | "Major subject: Physiology of Reproduction." Vita. |
| Physical Description: | viii, 43 leaves : graphs ; 28 cm |
| Bibliography: | Includes bibliographical references (leaves 34-36). |