Isolation and partial characterization of lymphocyte surface immunoglobulins from the channel catfish (Ictalurus punctatus, Rafinesque).

Bibliographic Details
Main Author: Eurell, Thomas Edward
Other Authors: Amoss, M. (degree committee member.), Grumbles, L. C. (degree committee member.), Haensly, W. E. (degree committee member.), Williams, J. D. (degree committee member.)
Format: Thesis Book
Language:English
Published: 1981.
Subjects:
Online Access:Link to ProQuest Copy
Link to OAKTrust copy

MARC

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099 |a 1981  |a Dissertation  |a E89 
100 1 |a Eurell, Thomas Edward. 
245 1 0 |a Isolation and partial characterization of lymphocyte surface immunoglobulins from the channel catfish (Ictalurus punctatus, Rafinesque). 
264 1 |c 1981. 
300 |a ix, 80 leaves :  |b illustrations ;  |c 29 cm 
336 |a text  |b txt  |2 rdacontent 
337 |a unmediated  |b n  |2 rdamedia 
338 |a volume  |b nc  |2 rdacarrier 
500 |a "Major subject: Veterinary Microbiology." 
500 |a Typescript (photocopy). 
500 |a Vita. 
502 |b Ph. D. in Philosophy  |c Texas A & M University  |d 1981 
504 |a Includes bibliographical references (leaves 71-79). 
520 3 |a Lactoperoxidase catalysed radioiodination, detergent lysis and specific immunoprecipitation were used to recover the surface immunoglobulins (SIg) from channel catfish lymphocyte sub-populations. The molecular weight and antigenic nature of blood, spleen and anterior kidney lymphocyte SIg were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography. These procedures revealed quantitative and qualitative differences between the lymphocyte sub-population SIg. Analysis of the serum immunoglobulin and lymphocyte SIg by SDS-PAGE showed that only one class of immunoglobulin heavy chain (analogous to mammalian (mu)-chain) was present and that the lymphocyte SIg (mu)-chain was approximately 3,700 daltons heavier than the (mu)-chain of catfish serum immunoglobulin. Autoradiographic analysis of the lymphocyte SIg showed that two immunoelectrophoretically distinct isotype components occurred on the surface of catfish blood lymphocytes. The SIg of spleen and anterior kidney lymphocytes do not appear to contain distinct isotype components. A possible differentiation sequence within the catfish lymphocyte sub-populations is hypothesized. 
650 0 |a Channel catfish.  |0 http://id.loc.gov/authorities/subjects/sh85022526 
650 0 |a Immune response.  |0 http://id.loc.gov/authorities/subjects/sh85064537 
650 0 |a Immunoelectrophoresis.  |0 http://id.loc.gov/authorities/subjects/sh85064557 
650 0 |a Immunoglobulins.  |0 http://id.loc.gov/authorities/subjects/sh85064568 
650 0 |a Lymphocytes.  |0 http://id.loc.gov/authorities/subjects/sh85079147 
650 4 |a Veterinary Microbiology. 
655 7 |a Academic theses.  |2 lcgft  |0 http://id.loc.gov/authorities/genreForms/gf2014026039 
700 1 |a Amoss, M.,  |e degree committee member. 
700 1 |a Grumbles, L. C.,  |e degree committee member. 
700 1 |a Haensly, W. E.,  |e degree committee member. 
700 1 |a Lewis, D. H.,  |e degree supervisor. 
700 1 |a Williams, J. D.,  |e degree committee member.  |0 http://id.loc.gov/authorities/names/n98045942 
710 2 |a Texas A & M University,  |e degree granting institution.  |0 http://id.loc.gov/authorities/names/n80125885 
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