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mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Molecular characterization of the patatin multigene family of potato
  name:
    name part: Mignery, Gregory Alan
    name part: 1958-
    role:
      role term: creator
  name:
    name part: Park, William D.
    role:
      role term: degree supervisor.
  name:
    name part: Gunn, J. Martyn
    role:
      role term: degree committee member.
  name:
    name part: Mullet, John E.
    role:
      role term: degree committee member.
  name:
    name part: Peterson, David O.
    role:
      role term: degree committee member.
  name:
    name part: Thomas, Terry L.
    role:
      role term: degree committee member.
  name:
    name part: Texas A & M University
    role:
      role term: degree granting institution.
  type of resource: text
  genre: bibliography
  genre: Academic theses
  origin info:
    place:
      place term: xx
    date issued: 1987
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xi, 84 leaves : illustrations (some folded) ; 29 cm
  abstract: A cDNA library was constructed from poly A mRNA isolated from membrane-bound polyribosomes of developing tubers. From this library, two groups of clones for the major tuber protein, patatin, were isolated. The complete sequence of two of these clones, pGM01 and pGM203, was determined. They are 98% homologous and correspond to the two major species of patatin identified by amino-terminal polypeptide sequence analysis. Both encode proteins of 363 amino acids with an additional 23 amino acid signal sequence. Patatin mRNA is approximately 1550 nucleotides in length and is present in large amounts in tubers, in trace amounts in roots, but is not present in leaves or stems. Potato DNA was used to construct genomic libraries from which 30 patatin genomic clones were isolated. All of these exhibited different restriction patterns, but could be divided into two classes based on the presence (Class II) or absence (Class I) of a 22 bp sequence within the 5'untranslated region. The complete DNA sequence of the Class I clone, PS20, the Class II clones SA10C and SB6B, and the 5' flanking sequence of seven additional clones was determined. The transcribed region of PS20 spans 3197 bp and is divided by six introns. The seven exons encode a transcript which is identical to the cDNA, pGM203. SB6B and SA10C both are pseudogenes which contain frameshift mutations. The 5' flanking sequences of both Class I and II patatin genes are highly homologous to position -87 and then diverge. This conserved region contains the CAAT and TATA homologies as well as a homology to the core enhancer sequence. Within a class there are additional large regions of homology, but differences exist that allow the patatin genes to be further divided into several discrete subclasses. S1 nuclease protection experiments with both Class I and Class II probes has shown that Class I transcripts are the predominant species present in tubers. Class II transcripts are present in tubers, but are 50-100 fold less abundan…
  note: Typescript (photocopy).
  note: Vita.
  note: "Major subject: Biochemistry."
  note: Ph. D. Texas A & M University 1987
  note: Includes bibliographical references (leaves 80-83).
  subject:
    topic: Molecular genetics
  subject:
    topic: Potatoes
    topic: Genetics
  subject:
    topic: Plant molecular genetics
  subject:
    topic: Major biochemistry
  identifier: http://gateway.proquest.com/openurl
  identifier: http://hdl.handle.net/1969.1/Dissertations-31773
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://hdl.handle.net/1969.1/Dissertations-31773
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://gateway.proquest.com/openurl
  record info:
    record content source: TXA
    record creation date: 880829
    record change date: 20190325090717.0
    record identifier: in00000024067
    language of cataloging:
      language term: eng