Table of Contents:
  • Mouse husbandry / Colin M. Hetherington
  • Isolation, culture and manipulation of pre-implantation mouse embryos / Hester P.M Pratt
  • Isolation, culture and manipulation of post-implantation mouse embryos / Rosa Beddington
  • Meiotic analysis in germ cells of man and the mouse / Ann C. Chandley
  • Karyotyping and sexing of gametes, embryos and fetuses and in situ hybridization to chromosomes / E.P. Evans
  • Cell marking techniques and their application / B.A.J. Ponder
  • Biochemical microassays for X-chromosome-linked enzymes HPRT and PGK / Marilyn Monk
  • Qualitative analysis of protein changes in early mouse development / Sarah K. Howlett
  • Construction of cDNA libraries for pre-implantation mouse embryos / Christine J. Watson and Josie McConnell
  • In situ hybridization to messenger RNA in tissue sections / Rebecca Haffner and Keith Willison
  • Production of transgenic mice / Nicholas D. Allen [and others]
  • Nuclear transplantation in fertilized and parthenogenetically activated eggs / S.C. Barton, M.L. Norris and M.A.H. Surani
  • The low temperature preservation of mouse oocytes and embryos / Maureen J. Wood, David G. Whittingham and William F. Rall
  • Fertilization of human oocytes and culture of human pre-implantation embryos in vitro / Peter R. Braude.