Fescue induced inhibition of prolactin secretion : development of a rat model /

Bibliographic Details
Main Author: Manning, Walter Scott, 1945-
Other Authors: Amoss, Max S. (degree committee member.), McArthur, Newell (degree committee member.), Scott, George G. (degree committee member.), Sis, Raymond F. (degree committee member.)
Format: Thesis Book
Language:English
Published: 1986.
Subjects:
Online Access:Link to ProQuest copy
Link to OAKTrust copy

MARC

Tag First Indicator Second Indicator Subfields
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035 |a (OCoLC)17625675 
035 |a (OCoLC)ocm17625675 
040 |a TXA  |b eng  |c TXA  |d OCLCQ  |d OCLCO  |d TXA 
049 |a TXAM 
099 |a 1986  |a Disser-  |a tation  |a M284 
100 1 |a Manning, Walter Scott,  |d 1945- 
245 1 0 |a Fescue induced inhibition of prolactin secretion :  |b development of a rat model / 
264 1 |c 1986. 
300 |a xiv, 194 leaves :  |b illustrations ;  |c 29 cm 
336 |a text  |b txt  |2 rdacontent 
337 |a unmediated  |b n  |2 rdamedia 
338 |a volume  |b nc  |2 rdacarrier 
500 |a "Major subject: Veterinary Anatomy." 
500 |a Typescript (photocopy). 
500 |a Vita. 
502 |b Ph. D.  |c Texas A & M University  |d 1986 
504 |a Includes bibliographical references (leaves 164-193). 
520 3 |a Effects of Kentucky-31 endophyte-infected seed extract (KEISE) on serum and plasma prolactin (PRL), on hypophyseal concentrations of PRL and the ultrastructure of hypophyseal and mammary glands were investigated. Timebred Sprague-Dawley rats received chronic levels of KEISE or PSS over a period of 10 d. The KEISE-treated dams did not produce a rise in serum PRL after whelping and were agalactic. Pups were either stillborn or small and weak and usually starved to death. PSS-treated dams produced normal, elevations in serum PRL after whelping, supplying copious amounts of milk to large, healthy litters. Student's T test indicated no difference between control and treated rats on d 16 and 13 and differences on d 20, 22, 24 and 26. Dunnett's test indicated treatment means for control rats differed from control means all days except d 16. Treatment means for KEISE-treated rats did not dif fer from control mean. Non-pregnant rats with heterogeneous hypophyseal grafts (HAG) were used to produce artificially high levels of PRL to observe the effects of KEISE on plasma PRL. A single dose of 4.86 gram seed equivalents/milliliter (g sd eg/ml) of KEISE produced a decrease in PRL for 3 to 6 h, then rebounded to the original zero levels. Plasma PRL in PSS-treated rats remained constant throughout the experiment. The pattern across time differed significantly (P<.05) A dose response using PRL and KEISE was observed in the HAG rats. Plasma PRL levels decreased as a linear function of increasing KEISE dose levels (30 min: P<0.03 and 60 min: P<0.01). Prolactin was immunocytochemically stained in hypophyseal tissue and evaluated with a microspectrophotometer. Absorbance in KEISE-treated animals was 22% while PSS-treated animals absorbed 37%, a substantial difference at the 95% confidence level. Hypophyseal and mammary tissues from KEISE and PSS-treated synchronized-bred nursing rats were studied ultrastructurally. PRL-producing cells from KEISE-treated rats showed decreased rough endoplasmic reticulum (RER), Golgi apparatus (GA), ribosomes and immature and mature secretory granules compared to control cells. The mammary gland in KEISE-treated rats contained noticeable differences in RER and GA when compared to controls and secretory vesicles increased confirming a lack of secretion. 
650 0 |a Cattle  |x Diseases. 
650 0 |a Forage plants  |x Toxicology. 
650 0 |a Prolactin. 
650 0 |a Tall fescue. 
650 4 |a Major veterinary anatomy. 
655 7 |a Academic theses  |2 lcgft 
700 1 |a Amoss, Max S.,  |e degree committee member. 
700 1 |a Bratton, Gerald,  |e degree supervisor. 
700 1 |a McArthur, Newell,  |e degree committee member. 
700 1 |a Scott, George G.,  |e degree committee member. 
700 1 |a Sis, Raymond F.,  |e degree committee member. 
710 2 |a Texas A & M University,  |e degree granting institution. 
856 4 1 |u http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=749099171&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD  |z Link to ProQuest copy  |t 0 
856 4 1 |u https://hdl.handle.net/1969.1/DISSERTATIONS-16948  |z Link to OAKTrust copy  |t 0 
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952 f f |p noncirc  |a Texas A&M University  |b J.J. Pickle Campus  |c High Density Repository  |s HDR  |d Remote Storage  |t 0  |e 1986 Dissertation M284  |h Other scheme  |i unmediated -- volume  |m A14839614639 
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